Functional domains of the p10 protein of Autographa californica nuclear polyhedrosis virus
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چکیده
منابع مشابه
Analysis of the promoter of the Autographa californica nuclear polyhedrosis virus p10 gene.
Functional analyses of the p10 gene promoter from the Autographa californica nuclear polyhedrosis virus (AcNPV) were performed by progressively deleting the 230 nucleotides upstream from the p10 coding sequences towards the ATG codon. Truncated promoter sequences retaining the full 5' non-coding leader of p10 were inserted in front of the chloramphenicol acetyltransferase (CAT) gene, and promot...
متن کاملIdentification of two independent transcriptional activation domains in the Autographa californica multicapsid nuclear polyhedrosis virus IE1 protein.
The Autographa californica multicapsid nuclear polyhedrosis virus immediate-early protein, IE1, is a 582-amino-acid phosphoprotein that regulates the transcription of early viral genes. Deletion of N-terminal regions of IE1 in previous studies (G. R. Kovacs, J. Choi, L. A. Guarino, and M. D. Summers, J. Virol. 66:7429-7437, 1992) resulted in the loss of transcriptional activation, suggesting th...
متن کاملThe pk-1 gene of Autographa californica multinucleocapsid nuclear polyhedrosis virus encodes a protein kinase.
Open reading frame (ORF) 9 of the EcoRI I fragment of the baculovirus Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) genome encodes a protein, PK-1, which has strong similarity to serine-threonine protein kinases. The published sequence of the pk-1 gene contains an error that, when corrected, extends the ORF by 228 nucleotides. Transcription of pk-1 was first detec...
متن کاملIdentifying the RNA polymerases that synthesize specific transcripts of the Autographa californica nuclear polyhedrosis virus.
Nuclear run-on assays carried out in the presence and absence of the RNA polymerase II inhibitor, alpha-amanitin, were used to determine the exact timing of the switch from inhibitor-sensitive transcription catalysed by host RNA polymerase II, to inhibitor-resistant transcription catalysed by the baculovirus-induced RNA polymerase. These studies revealed that the onset of alpha-amanitin-resista...
متن کاملExpression of foreign proteins on the surface of Autographa californica nuclear polyhedrosis virus.
Based on the method of direct cloning into the baculovirus genome by linearizing and re-ligation in presence of the target insert, we designed viral constructs that express foreign genes on the surface of baculovirus particles. We chose the glycosylated envelope protein gp41 of human immunodeficiency virus type 1 (HIV-1) as a model for displaying recombinant proteins on budded virus. The ectodo...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1993
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-74-4-563